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. Author manuscript; available in PMC: 2021 Aug 1.
Published in final edited form as: Am J Transplant. 2020 Mar 24;20(8):2206–2215. doi: 10.1111/ajt.15837

Figure 3: Donor-specific T cells deficient of FcγRIIB maintain ability to produce effector cytokines.

Figure 3:

WT OVA-specific CD45.2+ CD8+ T cells (WT OT-I) or Fcgr2b−/− OVA-specific CD45.2+ CD8+ T cells (Fcgr2b−/− OT-I) were harvested from the spleen and adoptively transferred into WT CD45.1+ C57BL/6 hosts that were then infected with Listeria. Animals were sacrificed and spleens were harvested on day 10 to assess cytokine response of the OT-I T cells. A) Representative flow plots of IFNγ- and TNF-producing OT-I T cells (splenocytes) on day 10 post adoptive transfer of either WT or Fcgr2b−/− OT-I T cells and subsequent infection with Listeria. B) Summary data of the frequency of IFNγ+TNF+ double-producing OT-I T cells and the frequency of single-producing IFNγ+TNF− OT-I T cells. Representative data from two independent experiments, n=4–5 mice per group. Mann-Whitney t test was performed, *p<0.05, ns=nonsignficant.