Table 3.
Evaluation of the effect of various nano-scaffolds on Leishmania major in the promastigote stage.
| Variable | MIC (%) | |||
|---|---|---|---|---|
| 24h | 48h | 72h | P value* | |
| CS-PEO | 40.7±2.53 | 55.3±2.15 | 62.7±4.6 | 0.001 |
| CS-PEO-berberine 0.5% | 48.3±2.08 | 60.2±2.32 | 66.0±3.4 | 0.001 |
| CS-PEO-berberine 1% | 54.9±3.5 | 65.0±1.98 | 71.1±2.13 | 0.04 |
| CS-PEO-berberine 2% | 59.0±2.13 | 67.6±2.82 | 73.9±3.12 | 0.001 |
| CS-PEO-berberine 3% | 66.2±3.81 | 71.5±4.6 | 76.2±3.08 | 0.001 |
| CS-PEO-berberine 4% | 76.0±5.23 | 82.2±3.54 | 88.0±1.88 | 0.001 |
| CS-PEO-berberine 5% | 78.9±2.87 | 83.0±3.2 | 90.0±4.07 | 0.001 |
| CS-PEO-berberine 6% | 84.6±2.23 | 91.0±1.2 | 98.1±1.17 | 0.001 |
| CS-PEO-berberine 8% | 95.7±0.9 | 99.1±0.7 | 100±0.0 | 0.001 |
| CS-PEO-berberine 10% | 97.5±0.93 | 100±0.0 | 100±0.0 | 0.001 |
| CS-PEO-berberine 15% | 100±0.0 | 100±0.0 | 100±0.0 | 0.001 |
| CS-PEO-berberine 20% | 100±0.0 | 100±0.0 | 100±0.0 | 0.001 |
*
One-way ANOVA tests, P<0.05 was considered significant; Data are expressed as mean±SD (n=3); CS-PEO: Chitosan -polyethylene oxide