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. Author manuscript; available in PMC: 2020 Aug 2.
Published in final edited form as: Biophys Chem. 1996 Nov 29;62(1-3):109–120. doi: 10.1016/s0301-4622(96)02199-0

Table 3.

Global anisotropy decay analysis of [Ru(bpy)2(Lx)] conjugated to HSA in the presence of various amounts of antibody a

Ratio Ab/Ag Ru(bpy)2(dcbpy)–HSA
ΘR1 = 36 ns; ΘR2 = 5115 ns
 Ru(bpy)2(mcbpy)–HSA
ΘR1 = 29 ns; ΘR2 = 3810 ns
 Ru(bpy)2(phen-ITC)–HSA
ΘR1 = 68 ns; ΘR2 = 7023 ns
g1r0 g2r0 Σgir0 g1r0 g2r0 Σgir0 g1r0 g2r0 Σgir0
0 0.173 0.008 0.181 0.144 0.011 0.155 0.102 0.0006 0.103
0.5 – – – 0.113 0.032 0.145 – – –
1.0 0.117 0.072 0.189 0.119 0.046 0.165 0.096 0.030 0.126
2.0 0.103 0.105 0.208 – – – 0.052 0.069 0.121
4.0 0.078 0.145 0.223 0.126 0.070 0.196 0.044 0.086 0.130
8.0 0.086 0.140 0.226 0.120 0.090 0.210 – – –
a

Excitation of 488 nm, emission above 580 nm, temperature 24°C.