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qPCR primer sequences for IR, IRS1, Akt, mTOR, and MBP.
| Gene | Forward (5′ ⟶ 3′) | Reverse (5′ ⟶ 3′) |
|---|---|---|
| IR | AGAGGTGGGCAATGTGACAG | ATGCGGTACCCAGTGAAGTG |
| IRS1 | ACCATCAGCAAGCAGGTCATTGT | CGGGTCCTCCACTTCACGAC |
| Akt | GGCAGGAGGAGGAGACGATGG | TTCATGGTCACACGGTGCTTGG |
| mTOR | AGAACCTGGCTCAAGTACGC | AGGATGGTCAAGTTGCCGAG |
| MBP | CTATAAATCGGCTCACAAGG | TGTGGGCGACTTCATCCT |
