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. 2020 Jul 22;2020:2685820. doi: 10.1155/2020/2685820

Figure 4.

Figure 4

PMSCs treatment increase Treg and decrease Th17 expression in CD4+ T cells from PBMC in vitro. (a) CD4+T cells were obtained from PBMC of rats at day 3 and 7 after UUO via microbeads, respectively, and cocultured with or without PBMCs. (b) A 0.4 μm transwell system was used and activated CD4+T cells were seeded in the lower chamber while PMSCs were seeded in the upper chamber. After 72 hours of incubation, CD4+T cells were stained with CD25-APC and Foxp3-FITC or IL17-APC, CD4-PE, respectively. (c) Histograms representation of the percentage of Treg cells (CD25+ Foxp3+) and IL17+ CD4+T cells in CD4+T cells cocultured with or without PMSC. Error bars are ±SD. ∗P < 0.05 compared with CD4+T cells group; ∗∗P < 0.01 compared with CD4+T cells group. n = 4 in each group.