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. 2020 Jul 22;2020:8980124. doi: 10.1155/2020/8980124

Figure 1.

Figure 1

Synergistic cytotoxic effect of Rg1 and TA3 on MG63 human osteosarcoma cells. (a) Rg1 chemical structure. (b) Chemical structure of Timosaponin AIII. (c) The viability of Rg1-treated MG63 cells was investigated by performing the viability assay using the CCK-8 kit. The cells were incubated with Rg1 (0, 100, 150, 200, 250, 300, and 400 μM) for 24 h. (d) Cytotoxic effect of Rg1 plus TA3 was monitored in MG63 cells. The cells were treated with each chemical compound for 24 h: TA3 (6 μM), Rg1 (250 μM), TA3 (6 μM) plus Rg1 (250 μM) for 24 h. Then the samples were subjected to the cell viability assay using CCK-8 kit. The results were statistically evaluated using ANOVA and Tukey's test (∗∗∗P < 0.001 vs control, $$P < 0.01 vs treatment of TA3, ##P < 0.01 vs treatment of Rg1).