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. 2020 Jul 22;2020:8980124. doi: 10.1155/2020/8980124

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Copyright © 2020 Sang Yeol Lee.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Synergistic effect of Rg1 and TA3 on caspase-3 activation. (a) Cellular ROS levels in MG63 cells incubated with TA3 (6 μM) and/or Rg1 (250 μM) were analyzed via the DCF-DA method. ROS levels were not changed significantly by the treatment of TA3 and/or Rg1. (b) Quantifications of DCF-DA ROS assay. (c) The level of the uncleaved form of caspase-3 decreased further when the cells were treated with both Rg1 (250 μM) and TA3 (6 μM) than with TA3 (6 μM) alone while the uncleaved form of caspase-7 was not further affected by Rg1 (250 μM) and TA3 (6 μM) together than TA3 (6 μM) alone.