Figure 2.

Effect of trehalose on p62 mRNA and protein expression levels and p62 turnover. (a, e) Trehalose upregulated p62 expression at the transcription and protein levels. (a–d) Immunoblotting with p62 antibodies in whole-cell lysate from treated cells. (e) RNA extraction for RT-qPCR to evaluate the gene expression level of p62. (b) Trehalose increased p62 in the presence of chloroquine (CQ). Data represent the mean ± SD of 3 independent experiments. Statistical analysis was performed by the unpaired t test or one-way ANOVA followed by multiple comparison tests. ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001 vs. control.