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Accumulation of p62 by trehalose was not due to impaired proteasome degradation. (a, b) Time-dependent increase in p62 protein level by MG132 in cell lysate using immunoblot. (c) Proteasome activity level in whole-cell lysates from cells. (d) Trehalose increased p62 level in the presence of MG132. Data represent the mean ± SD of 3 independent experiments. Statistical analysis was performed by one-way ANOVA followed by multiple comparison tests. ∗p < 0.05, ∗∗p < 0.001 vs. control.
