Figure 7.

Cytoprotection of trehalose against oxidative damage by hydroquinone (HQ) in ARPE-19 cells. (a) Trypan blue assay reveals that trehalose is nontoxic over a wide dose range. (b) Trehalose decreased basal level of reactive oxygen species (ROS) assessed using CM-H₂DCFDA assay (c, d) Trehalose pretreatment improves the viability of cells and lowered the protein carbonyl levels in cells exposed to HQ. Data represent the mean ± SD of 3 independent experiments. Statistical analysis was performed by one-way ANOVA test, followed by multiple comparison post hoc test; ^∗p < 0.05, ^∗∗∗p < 0.001.