Figure 2.
TD-PCR F3-B3 for amplifying S. bovis NADH-1. A 58-53°C temperature range and 53°C × 15 cycles were used. Sm: S. mansoni DNA; Sh: S. haematobium DNA; Sj: S. japonicum DNA; Si: S. intercalatum DNA; Sb: S. bovis DNA; N: negative control (ultrapure water, no DNA). M: molecular weight marker (100 bp PLUS BLUE DNA ladder).