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TD-PCR F3-B3 for amplifying genus Schistosoma ITS-1. A 61-57°C temperature range, and 57°C × 30 cycles were used. Sm: S. mansoni DNA; Sh: S. haematobium DNA; Sj: S. japonicum DNA; Si: S. intercalatum DNA; Sb: S. bovis DNA; N: negative control (ultrapure water, no DNA). M: molecular weight marker (100 bp PLUS BLUE DNA ladder).
