(a) LAMP primers for the S. bovis sequence (LAMP Designer)
| NADH subunit 1 | ||||||
|---|---|---|---|---|---|---|
| Primer | 5′ pos | 3′ pos | Length (bp) | Tm (°C) | GC ratio (%) | Sequence |
| SbF3 | 219 | 236 | 18 | 56.8 | 38.9 | TTCATTGTTAGGTTGCGT |
| SbB3 | 642 | 619 | 24 | 57 | 33.3 | TCTATATTCTACTCTAATCCCTCT |
| SbFIP | 48 | TCAGTATCATCTCAAACATCACACTAGTAGTATGTTCTGTCTTAAGTT | ||||
| SbBIP | 45 | TTTGTAGTACCTCTGGTTTACATCATTCACTCTCAGACTCTACAT | ||||
| SbF2 | 327 | 349 | 23 | 56.8 | 30.4 | AGTAGTATGTTCTGTCTTAAGTT |
| SbF1c | 424 | 448 | 25 | 62.1 | 36 | TCAGTATCATCTCAAACATCACACT |
| SbB2 | 593 | 612 | 20 | 57 | 40 | TTCACTCTCAGACTCTACAT |
| SbB1c | 520 | 544 | 25 | 61.8 | 36 | TTTGTAGTACCTCTGGTTTACATCA |
| SbLF | 364 | 388 | 25 | 61.9 | 36 | ACTTAGACCATGAACATCAACCTAT |
| SbLB | 560 | 584 | 25 | 61.9 | 40 | TACTAAGTGAGAGTAATCGAACACC |