(b) LAMP primers for the genus Schistosoma sequence (Primer Explorer V5)
| ITS-1 | ||||||
|---|---|---|---|---|---|---|
| Primer | 5′ pos | 3′ pos | Length (bp) | Tm (°C) | GC ratio (%) | Sequence |
| SF3 | 2 | 19 | 18 | 59.7 | 61 | TTGACCGGGGTACCTAGC |
| SB3 | 200 | 218 | 19 | 59.5 | 53 | CGTGAATGGCAAGCCAAAC |
| SFIP | 39 | ATCGCCCTTGGCAGATCAGGCTGTCGTATGCCCTGATGG | ||||
| SBIP | 40 | ATATGCATGCAAATCCGCCCCGCGGATCGCTTCAACAGTGTA | ||||
| SF2 | 20 | 38 | 19 | 59.2 | 58 | CTGTCGTATGCCCTGATGG |
| SF1c | 61 | 80 | 20 | 64.2 | 60 | ATCGCCCTTGGCAGATCAGG |
| SB2 | 180 | 199 | 20 | 59.5 | 50 | CGGATCGCTTCAACAGTGTA |
| SB1c | 135 | 156 | 20 | 65.9 | 55 | ATATGCATGCAAATCCGCCCCG |
| SLF | 39 | 60 | 22 | 60.4 | 45 | CAGATCAGGCAACCCGAAAG |
For S. bovis (Sb) and genus Schistosoma (S): F3=forward outer primer; B3=backward outer primer; FIP=forward inner primer (comprising F1c and F2 sequences); BIP=backward inner primer (comprising B1c and B2 sequences); LF=loop forward primer; LB=loop backward primer.