Table 2. Samples successfully identified as P. canaliculus in each experiment.
Numbers in parenthesis relate to number of samples tested, and are followed by the number of samples that were successfully amplified.
| Mitochondrial Amplicons | Nuclear Amplicon | ||||
|---|---|---|---|---|---|
| Collection (month) | Pcan(305 bp) | qPCR Pcan(305 bp) | COX1(191 bp) | Chitin Synthase(198 bp) | |
| Aged shells | 0 (n = 10) | 8 | (n = 10) 9 | 9 | 8 |
| 1 (n = 10) | 10 | (n = 9) 9 | 10 | 7 | |
| 2 (n = 10) | 9 | 9 | – | ||
| 4 (n = 10) | 7 | 9 | – | ||
| 5 (n = 8) | 8 | 8 | – | ||
| 6 (n = 10) | 10 | (n = 9) 9 | 10 | 1 | |
| 7 (n = 10) | 10 | 10 | – | ||
| 8 (n = 10) | 10 | 10 | – | ||
| 9 (n = 10) | 10 | 10 | – | ||
| 10 (n = 10) | 10 | 10 | – | ||
| 11 (n = 10) | 10 | 10 | – | ||
| 12 (n = 10) | 10 | 10 | – | ||
| 13 (n = 10) | 10 | (n = 8) 8 | 10 | 3 | |
| N total = 128 | Average= 95.3% | Average= 97.2% | Average= 97.7% | Average= 47.5% | |