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Positive sample serial dilution.
| Sample Dilution | Expected N1 CT | Actual N1 CT | Expected N2 CT | Actual N2 CT |
|---|---|---|---|---|
| Neat | 30.8 | 31.9 | 30.3 | 30.9 |
| 1:10 (1:40) | 35.4 | 35.4 | 34.9 | 35.3 |
| 1:100 (1:400) | 38.7 | NDET | 38.2 | NDET |
| 1:1,000 (1:4,000) | 42.0 | NDET | 41.5 | NDET |
Abbreviations: CT, cycle threshold, NDET, not detected, N1 and N2 are SARS-CoV-2 nucleocapsid gene targets for PCR. Parentheticals denote respective pooling dilutions.
