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. Author manuscript; available in PMC: 2020 Dec 5.
Published in final edited form as: Science. 2020 Jun 5;368(6495):1132–1135. doi: 10.1126/science.aay5134

Fig. 2. NELL2 is required for ADAM3 maturation and subsequent sperm fertilizing ability.

Fig. 2.

(A-F) Migration of GFP-tagged WT (A-C) and Nell2 KO (D-F) sperm ejaculated into WT female reproductive tract. (G, H) Sperm-ZP binding assay with WT (G) and Nell2 KO (H) spermatozoa. Bars, 100 μm. (I) Immunoblot detection of cauda epididymal sperm lysates. (J) Immunoblot detection of ADAM3 in TGC, TS (testicular spermatozoa), caput, corpus, and cauda epididymal spermatozoa. (K) Expression of genes encoding proteases in WT and Nell2 KO caput epididymis. Only genes whose average read counts in WT are >100 are shown. (L) Immunoblot analysis of caput epididymal protein lysates from WT, Nell2 KO, and Ros1 KO males.