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. 2020 Jul;8(13):823. doi: 10.21037/atm-19-3850

Figure 5.

Figure 5

DB restored the increase of Neu1 in APAP-induced hepatotoxicity. (A) Protein levels of Neu1 were decreased in APAP-treated HepG2 cells (n=3) and mice (n=8) treated with DB by Western Blot. (B) Relative density of Neu1 in liver of APAP-treated mice by Western Blot. (C and D) Transcript levels of Neu1 were decreased in APAP-treated HepG2 cells (n=4) and mice (n=6–8) treated with DB by qRT-PCR. (E and F) Enzyme activity of Neu1 were inhibited in APAP-treated HepG2 cells and mice treated with DB (n=6–8). (G) Total cell lysate and liver lysates were subjected to Western blot analysis for LAMP1 (n=4). (H) lysosome marker was examined by immunohistochemistry. LAMP1 was increased in DB treated mice (n=3), scale bar =50 µm. *, P<0.05; **, P<0.005; ***, P<0.001.