Figure 2 [color]: Single-cell RNA sequencing of infant and adult RPE-choroid samples from 8 human donors.

A. After single-cell RNA sequencing, UMAP dimensionality reduction was performed to visualize clusters of choroidal cell types. A total of 37,070 cells were recovered after filtering, and all major choroidal cell types were identified. B. Cells comprising the endothelial cell clusters (dashed box in A) were well represented from both adult (left) and infant (right) libraries. C. In each cell type, we identified the 100 most enriched genes, averaged gene expression across cells, and performed hierarchical clustering analysis on these values (left). Violin plots (right) indicate relative expression levels of previously reported cell-specific genes within all cells of each cluster.