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. Author manuscript; available in PMC: 2020 Dec 29.
Published in final edited form as: Nat Methods. 2020 Jun 29;17(8):844–851. doi: 10.1038/s41592-020-0883-z

Extended Data Fig. 1 |. Azide probes.

Extended Data Fig. 1 |

a-b, A single azide band at 2098 cm−1 can be consistently detected on azido-palmitic acid labeled (a) and AHA labeled (b) but not on unlabeled control inside Raw264.7 cells. c, High-definition imaging on single azido-palmitic acid labeled Raw264.7 cells. Pixel size = 0.66 μm. The diffraction-limited resolution is about ~3 μm for the azide band. d, Imaging on C. elegans labeled with azido-palmitic acid. Pixel size = 3.3 μm. Experiments in a-c were repeated five times independently with similar results. Experiments in d were repeated three times independently with similar results. Scale bars, 5 μm in c, 100 μm in d.