Table 1. Properties of Janelia Fluor dyes 1–11, 13–20, and 37–38.

All properties were measured in 10 mM HEPES, pH 7.3 except for K_L–Z, which was determined in 1:1 dioxane:H₂O. Properties for 2, 4, 5, 9, 10, 13, and 14were taken from previous work^{3, 4, 7}. ND: not determined due to low absorbance.

Compound	Name	X	Y	Z	NR2	λabs (nm)	ε (M–1cm–1)	λem (nm)	Φ	KL–Z
1	JF502		H	H		502	57,800	533	0.71	4.33
2	JF549		H	H		549	101,000	571	0.88	3.47
3	JF570		H	H		570	83,600	593	0.63	2.24
4	JF608		H	H		608	99,000	631	0.67	0.091
5	JF646		H	H		646	5,600	664	0.54	0.0014
6	JF668		H	H		668	26,700	687	0.34	0.0093
7	–		H	H		~704	<200	~723	ND	<0.0001
8	–		H	H		ND	<100	ND	ND	<0.0001
9	JF525		H	H		525	94,000	549	0.91	0.068
10	JF585		H	H		585	1,500	609	0.78	~0.001
11	JF479		H	H		479	47,900	517	0.62	2.88
13	JF552		F	H		552	95,000	575	0.83	0.70
14	JF526		F	H		526	19,000	550	0.87	0.005
15	JF669		H	F		669	112,000	682	0.37	0.262
16	JF690		H	F		690	150,000	707	0.24	2.90
17	JF722		H	F		722	87,200	743	0.11	0.026
18	JF724		H	F		724	6,600	748	0.05	~0.001
19	JF571		H	F		571	101,000	590	0.78	7.93
20	JF593		H	F		593	90,300	612	0.55	6.06
37	JF559		H	F		559	106,000	579	0.85	6.22
38	JF711		H	F		711	12,400	732	0.17	~0.001