Fig. 4.
FLIO imaging in the healthy retina. A) Representative fundus autofluorescence intensity (FAF) and mean fluorescence lifetime image (FLIO; long spectral channel (LSC) 560–720 nm; color range Tm: 200–1000 ps) of the right eye of a 35 years old healthy subject. For comparability, the same settings (LSC, consistent color range if not further specified) is used for further illustration in retinal pathologies. B) Calculated fluorescence decay curves over the time interval of 12 ns for individual pixel locations (1–4) marked in A). C) 2D histogram showing the distribution of fluorescence lifetime clouds for specific retinal areas when plotting the short decay component T1 (x-axis) versus the long decay component T2 (y-axis) according to equation (3). Note the different time scales used for the axis.