. 2020 Jun 10;13(6):1037–1044. doi: 10.14202/vetworld.2020.1037-1044

Table-2.

Primers sequences, target genes, amplicon sizes, and cycling conditions.

Target gene	Primers sequences	Amplified segment (bp)	Primary denaturation	Amplification (35 cycles)			Final extension	Reference

				Secondary denaturation	Annealing	Extension
bla_TEM	ATCAGCAATAAACCAGC	516	94°C 5 min	94°C 30 sec	54°C 40 sec	72°C 45 sec	72°C 10 min	[18]
bla_TEM	CCCCGAAGAACGTTTTC	516	94°C 5 min	94°C 30 sec	54°C 40 sec	72°C 45 sec	72°C 10 min
bla_SHV	AGGATTGACTGCCTTTTTG	392	94°C 5 min	94°C 30 sec	54°C 40 sec	72°C 40 sec	72°C 10 min
bla_SHV	ATTTGCTGATTTCGCTCG	392	94°C 5 min	94°C 30 sec	54°C 40 sec	72°C 40 sec	72°C 10 min
bla_OXA-1	TCAACTTTCAAGATCGCA	609	94°C 5 min	94°C 30 sec	54°C 40 sec	72°C 45 sec	72°C 10 min
bla_OXA-1	GTGTGTTTAGAATGGTGA	609	94°C 5 min	94°C 30 sec	54°C 40 sec	72°C 45 sec	72°C 10 min
ampC	TTCTATCAAMACTGGCARCC	550	94°C 5 min	94°C 30 sec	50°C 40 sec	72°C 45 sec	72°C 10 min	[19]
ampC	CCYTTTTATGTACCCAYGA	550	94°C 5 min	94°C 30 sec	50°C 40 sec	72°C 45 sec	72°C 10 min	[19]
bla_CTX-M	ATG TGC AGY ACC AGT AAR GTK ATG GC TGG GTR AAR TAR GTS ACC AGA AYC AGC GG	593	94°C 5 min	94°C 30 sec	54°C 40 sec	72°C 45 sec	72°C 10 min	[20]