Table-3.

Antibacterial activity of herbal formula against mastitis bacteria in comparison with standard antibiotic.

Sterilization Method	Herbal Formula	Mean inhibition zones (mm) of microorganism
		Escherichia coli	Staphylococcus aureus
Extract without sterilization (control)	A	9.7	22.3
	B	10.0	22.7
	C	10.0	23.2
Extract sterilized with syringe filter	A	3.3	19.7
	B	3.3	21.3
	C	2.7	19.0
Extract sterilized with autoclave	A	9.5	20.7
	B	9.7	21.7
	C	9.7	20.7
Standard antibiotic	TE-30	23.8	22.7
	E-15	12.2	28.1
	P-10	1.0	21.1

Sterilization methods applied were sterilization using syringe filter 0.45 μM and sterilization using autoclave at 121°C for 15 min