Table-1.

Primer sets used to amplify fragments of 16S rRNA, enterotoxins, and virulence factors genes in S. aureus.

Primer Name	Sequence (5’-3’)	Amplified product size (bp)	Annealing Temperature	No. of cycles	Reference
16S rRNA	GTA GGT GGC AAG CGT TAT CC CGC ACA TCA GCG TCA G	228	50°C/50 s	40 cycles	[16]
sea	GCA GGG AAC AGC TTT AGG C GTT CTG TAG AAG TAT GAA ACA CG	521	48°C/1 min 44°C/1 min (reaction A)	15 cycles
seb	ACA TGT AAT TTT GAT ATT CGC ACT G TGC AGG CAT CAT GTC ATA CCA	667		20 cycles	[17]
sec	CTT GTA TGT ATG GAG GAA TAA CAA TGC AGG CAT CAT ATC ATA CCA	284			[16]
sed	CCA ATA ATA GGA GAA AAT AAA AG ATT GGT ATT TTT TTT CGT TC	278	57°C/2 min (reaction B)	35 cycles	[18]
see	AGG TTT TTT CAC AGG TCA TCC CTT TTT TTT CTT CGG TCA ATC	209			[19]
tsst	GCT TGC GAC AAC TGC TAC AG TGG ATC CGT CAT TCA TTG TTA T	559	50°C/50 s	40 cycles	[16]
nuc	GCG ATT GAT GGT GAT ACG GTT AGC CAA GCC TTG ACG AAC TAA AGC	270	of 50°C/1 min	40 cycles	[20]
mecA	AAA ATC GAT GGT AAA GGT TGG C ATC TGT ACT GGG TTA ATC	533	of 50°C/1 min	40 cycles	[21]

S. aureus: Staphylococcus aureus