Table-5.
The mean±standard deviation of PCR cycle number at which the sample’s reaction curve intersects the threshold line in rumen fluid and feces.
| Primer sequences | The mean PCR cycle number | ||||
|---|---|---|---|---|---|
| Rumen fluid (n=5) | p-value | Feces (mixed sample from five samples) | |||
| CoG | SynG | CoG | SynG | ||
| Total prokaryotes (V3) (bacteria+archaea) | 10.5±0.84 | 12.3±1.41 | 0.032* | 33.9 | 13.3 |
| Total methanogens (Met630/803) | 31.5±1.75 | 27.8±2.16 | 0.049* | 23.5 | 26.6 |
| M. stadtmanae Stad. | 17.5±1.86 | 18.1±0.82 | 0.857 | 8.8 | - |
| M. ruminantium Rum16S | 18.3±5.41 | 22.7±0.71 | 0.400 | 17.0 | 19.4 |
| Methanobrevibacter smithii Smit16S | 13.4±4.35 | 17.8±5.82 | 0.699 | 10.5 | 12.7 |
*
Significant at p<0.05. CoG=Control group, SynG=Synbiotic group