Table-1.
Primers used for PCR amplification of tumor viruses.
| Gene | Primer sequence | Annealing temp. | Fragment size | Reference |
|---|---|---|---|---|
| ALV A | H5-F GGATGAGGTGACTAAGAAAG EnvA- RAGAGAAAGAGGGGYGTCTAAGGAGA |
48 | 694 | [23] |
| ALV-B and D | BD-F CGAGAGTGGCTCGCGAGATGG BD-R AGCCGGACTATCGTATGGGGTAA |
52 | 1100 | [24] |
| ALV-C | C-F CGAGAGTGGCTCGCGAGATGG C-R CCCATATACCTCCTTTTCCTCTG |
52 | 1400 | [25] |
| ALV-J | H5-F GGATGAGGTGACTAAGAAAG H7-R CGAACCAAAGGTAACACACG |
48 | 545 | [26] |
| MDV | ICP4 F GGATCGCCCACCACGATTACTACC ICP4 R ACTGCC TCACACAACCTCATC TCC |
58 | 318 | [27] |
| REV | env-F AGCTAGGCTCGTATGAA env-R TATTGACCAGGTGGGTTG |
48 | 438 | [28] |
PCR=Polymerase chain reaction, ALV=Avian leukosis virus