Table-3.
Validation parameters for HPLC.
| Sl. no. | Parameter | Values for oxytetracycline |
|---|---|---|
| 1. | Retention time (min) | 5.4 |
| 2. | Linear regression equation | y=0.0003x−0.0046 |
| 3. | R2 | 0.9995 |
| 4. | Linear range (ng/mL) | 50-2000 |
| 5. | LOD (ng/mL) | 82.65 |
| 6. | LOQ (ng/mL) | 250.45 |
| 7. | Intra-assay precision (RSD %) n=6 (ng/mL) | |
| 200 | 4.10 | |
| 1000 | 6.80 | |
| 2000 | 2.82 | |
| 8. | Inter-assay precision (RSD %) n=6 (ng/mL) | |
| 200 | 7.61 | |
| 1000 | 0.14 | |
| 2000 | 3.37 | |
| 9. | Accuracy (recovery %) n=6 (ng/mL) | |
| 200 | 90.19 | |
| 1000 | 88.65 | |
| 2000 | 94.75 | |
HPLC=High-performance liquid chromatography, LOD=Limit of detection, LOQ=Limit of quantitation, RSD=Relative standard deviation