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. 2020 Jun 30;13(6):1234–1244. doi: 10.14202/vetworld.2020.1234-1244

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Copyright: © Abou-Elkhair, et al.

Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Figure-2 — Effect of amino acid supplementation in a low-protein diet on the mRNA expression of cecal and splenic interleukin-4 (IL4) and interferon-gamma (INFγ) of broiler chickens at the end of the experiment (35 days of age). Data are presented as the mean±standard error of the mean. Bars not sharing a common letter are different (p<0.05). The experimental diets were formulated to contain 22, 21, and 20 % crude protein (CP) for the starting period, 20, 19, and 18% CP for the growing period, and 18, 17, and 16% CP for the finishing period in control, CP-1%, and CP-2%, respectively. The CP-1% and CP-2% groups were supplemented with threonine, valine, and tryptophan to meet or exceed the respective levels of the control diet.