Table-1.
Composition of the experimental diets (as fed basis).
| Items, g/kg | Starter (days 1 to 14) | Grower (days 15 to 28) | Finisher (days 29 to 35) | ||||||
|---|---|---|---|---|---|---|---|---|---|
| CON | CP-1% | CP-2% | CON | CP-1% | CP-2% | CON | CP-1% | CP-2% | |
| Yellow corn | 598 | 619 | 642 | 638 | 660 | 681 | 680 | 706 | 730 |
| SBM, 46% CP | 342 | 329 | 312 | 308 | 290 | 272 | 270 | 245 | 220 |
| Corn gluten | 20.0 | 10.0 | 0.0 | 10.0 | 5.0 | 0.0 | 0.0 | 0.0 | 0.0 |
| Vegetable oil | 3.0 | 3.0 | 3.0 | 10.0 | 9.0 | 8.0 | 17.0 | 14.0 | 12.0 |
| Limestone | 15.5 | 15.5 | 15.5 | 14.5 | 14.5 | 14.5 | 14 | 14.0 | 14.0 |
| Monocalcium phosphate | 10.0 | 10.0 | 10.0 | 9.0 | 9.0 | 9.0 | 8.0 | 8.0 | 8.0 |
| L-Lysinea | 1.7 | 2.2 | 3.0 | 1.3 | 2.0 | 2.8 | 1.1 | 1.8 | 3.05 |
| DL-Methionineb | 1.4 | 1.5 | 3.0 | 1.5 | 1.7 | 3.0 | 1.4 | 1.5 | 2.68 |
| L-Threonine | 0.0 | 0.79 | 1.58 | 0 | 0.50 | 0.99 | 0.0 | 0.35 | 0.7 |
| L-Valine | 0.0 | 0.04 | 0.076 | 0 | 0.11 | 0.22 | 0.0 | 0.025 | 0.05 |
| Tryptophan | 0.0 | 0.05 | 0.1 | 0 | 0.045 | 0.099 | 0.0 | 0.09 | 0.18 |
| Common salt | 2.0 | 2.0 | 2.0 | 2.5 | 2.5 | 2.5 | 3.0 | 3.0 | 3.0 |
| Premixc | 2.0 | 2.0 | 2.0 | 2.0 | 2.0 | 2.0 | 2.0 | 2.0 | 2.0 |
| Na-bicarbonate | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 |
| Choline | 0. 90 | 0. 90 | 0. 90 | 0.50 | 0.50 | 0.50 | 0.50 | 0.50 | 0.50 |
| Mycotoxin | 0.50 | 0.50 | 0.50 | 0.50 | 0.50 | 0.50 | 0.50 | 0.50 | 0.50 |
| Avizyme | 0.20 | 0.20 | 0.20 | 0.20 | 0.20 | 0.20 | 0.20 | 0.20 | 0.20 |
The experimental diets were formulated to contain 22, 21, and 20 % CP for the starting period, 20, 19, and 18% CP for the growing period, and 18, 17, and 16% CP for the finishing period in CON, CP-1%, and CP-2%, respectively. The CP-1% and CP-2% groups were supplemented with threonine, valine, and tryptophan to meet or exceed the respective levels of the control diet.
L-Lysine, lysine monohydrochloride by Feed Grade 99%.
DL-Met, Met AMINO (DL-2-amino-4-(methyl-thio)-butane acid, DL-Met, a-amino-Y-methyl-oily acid) by Feed Grade 99%.
Supplied per kilogram of diet: Vitamin A, 12,000 IU; Vitamin D3, 3,000 IU; Vitamin E, 40 mg; Vitamin K3, 3 mg; Vitamin B1, 2 mg; Vitamin B2, 6 mg; Vitamin B6, 5 mg; Vitamin B12, 0.02 mg; niacin, 45 mg; biotin, 0.075 mg; folic acid, 2 mg; pantothenic acid, 12 mg; Mn, 100 mg; Zn, 600 mg; Fe, 30 mg; Cu, 10 mg; I, 1 mg; Se, 0.2 mg; Co, 0.1 mg. d Each kg of Avizyme contains 4 g phytase, 40 g xylanase, 15 g amylase, 2 g pectinase, 8 g protease, 40 g beta glucanase, 40 g cellulase, 2.5 g methyl paraben, 1 g propyl paraben, and 847.5 g calcite