Table-4.
Oligonucleotide sequence of growth- and immune-related gene primers [19].
| Genea | Sequence (5’®3’) | Annealing temperature (°C) | Accession Number | Product size (bp) | E, %b |
|---|---|---|---|---|---|
| IL-4 | F-AATGACATCCAGGGAGAGGTTTC R-GCTAGTTGGTGGAAGAAGGTACG |
55 | JN639847 | 219 | 86 |
| INF-γ | F-AGCTGACGGTGGACCTATTATTGT R-CGGCTTTGCGCTGGATTC |
58 | JN942588 | 260 | 86 |
| 28S rRNA | F-CAGGTGCAGATCTTGGTGGTAGTA R- GCTCCCGCTGGCTTCTCC |
58 | JN639848 | 273 | 98 |
a
IL-4=Interleukin 4, IFN-γ=Interferon gamma; 28S rRNA=28 S ribosomal RNA,
b
efficiency (E) was calculated from the slope of the standard curve using the equation: E=10 (−1/slope)