Table-7.

Effect of amino acid supplementation in a low-protein diet on blood biochemical constituents of broiler chickens at the end of the experiment (35 days of age).

Itemb	Treatmentsa			SEM	p-value
	CON	CP-1%	CP-2%		CON versus treatment	Linear	Quadratic
Total protein, g/dL	4.18b	4.50b	5.06a	0.124	<0.001	<0.001	0.29
Albumin, g/dL	3.06	3.16	3.40	0.153	0.12	0.05	0.61
Globulin, g/dL	1.12b	1.34ab	1.66a	0.120	0.003	0.001	0.64
A:G	2.79	2.41	2.08	0.278	0.08	0.03	0.92
Total cholesterol, mg/dL	207.0	205.8	206.7	1.81	0.61	0.34	0.85
Total triglycerides, mg/dL	200.8a	197.2a	192.4b	1.58	0.001	<0.001	0.67
Glucose, mg/dL	174.8	176.2	175.8	2.179	0.80	0.65	0.64
Uric acid, mg/dL	6.56a	5.52b	5.60b	0.311	0.01	0.009	0.06
Creatinine, mg/dL	0.520a	0.530a	0.468b	0.182	0.01	0.01	0.04
GOT, U/dL	77.00a	75.00a	71.80b	1.013	0.001	<0.001	0.51
GPT, U/dL	69.00a	67.80ab	65.70b	0.959	0.02	0.006	0.64
Alkaline phosphatase, U/L	12.34a	11.22ab	11.14b	0.424	0.03	0.02	0.18
Phagocytic index	3.57b	3.68b	4.84a	0.342	0.005	0.003	0.10
Phagocytic activity	61.80b	65.80ab	71.40a	2.372	0.006	0.002	0.70

^a,bMeans within rows with different letters are different at p<0.05; Tukey’s tests were applied to compare means,

SEM = Standard error of the mean.

^aThe experimental diets were formulated to contain 22, 21, and 20% CP for the starting period, 20, 19, and 18% CP for the growing period, and 18, 17, and 16% CP for the finishing period in CON, CP-1%, and CP-2%, respectively. The CP-1% and CP-2% groups were supplemented with threonine, valine, and tryptophan to meet or exceed the respective levels of the control diet.

^bA:G=Albumin:globulin; GPT=Alanine aminotransferase; GOT=Aspartate aminotransferase