Figure 2.

The effect of circ_0007385 knockdown on NSCLC cell proliferation, migration and invasion in vitro. (a–f) A549 and H1975 cells were transfected with shRNA targeting circ_0007385 (sh‐circ) or the negative control sh‐NC for 48 hours. (a) RT‐qPCR confirmed the circ_0007385 level () sh‐NC, () sh‐circ. (b and c) CCK‐8 assessed optical density value at 450 nm (OD450 nm value) at 24, 48 and 72 hours () sh‐NC, () sh‐circ; () sh‐NC, () sh‐circ. (d and e) Transwell assays determined the number of migratory and invasive cells () sh‐NC, () sh‐circ; () sh‐NC, () sh‐circ. (f) Western blotting detected protein expression of the proliferating cell nuclear antigen (PCNA), E‐cadherin (E‐cad) and N‐cadherin (N‐cad) () sh‐NC, () sh‐circ; () sh‐NC, () sh‐circ. **P < 0.01.