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. 2020 Jun 29;11(8):2196–2208. doi: 10.1111/1759-7714.13527

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© 2020 The Authors. Thoracic Cancer published by China Lung Oncology Group and John Wiley & Sons Australia, Ltd

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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The effect of circ_0007385 knockdown on cisplatin (DDP) sensitivity of NSCLC cells in vitro. (a–f) A549 and H1975 cells were transfected with sh‐circ or sh‐NC, and then treated with DDP for 48 hours. (a and b) CCK‐8 measured the inhibition rate (%) of cell viability after treatment of 0, 2.5, 5, 10, 20, 40, and 80 μM of DDP () sh‐NC IC50 = 10.39, () sh‐circ IC50 = 5.92; () sh‐NC IC50 = 13.69, () sh‐circ IC50 = 7.77. IC50, half maximal inhibitory concentration. (c and d) Flow cytometry (FCM) and Annexin V fluorescein isothiocyanate (FITC) apoptosis detection kit examined apoptosis rate (%) after treatment of 10 μM of DDP. (e and f) FCM and fluorescein active caspase‐3 staining kit examined relative caspase‐3 activity after treatment of 10 μM of DDP. **P < 0.01.

Inline graphic — The effect of circ_0007385 knockdown on cisplatin (DDP) sensitivity of NSCLC cells in vitro. (a–f) A549 and H1975 cells were transfected with sh‐circ or sh‐NC, and then treated with DDP for 48 hours. (a and b) CCK‐8 measured the inhibition rate (%) of cell viability after treatment of 0, 2.5, 5, 10, 20, 40, and 80 μM of DDP () sh‐NC IC50 = 10.39, () sh‐circ IC50 = 5.92; () sh‐NC IC50 = 13.69, () sh‐circ IC50 = 7.77. IC50, half maximal inhibitory concentration. (c and d) Flow cytometry (FCM) and Annexin V fluorescein isothiocyanate (FITC) apoptosis detection kit examined apoptosis rate (%) after treatment of 10 μM of DDP. (e and f) FCM and fluorescein active caspase‐3 staining kit examined relative caspase‐3 activity after treatment of 10 μM of DDP. **P < 0.01.