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. 2020 Jun 29;11(8):2196–2208. doi: 10.1111/1759-7714.13527

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© 2020 The Authors. Thoracic Cancer published by China Lung Oncology Group and John Wiley & Sons Australia, Ltd

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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The target relationship between circ_0007385 and miRNA (miR)‐519d‐3p. (a) The StarBase algorithms showed the complementary binding sequence of miR‐519d‐3p on the wild‐type of circ_0007385 (circ_0007385 WT). The mutant type of circ_0007385 (circ_0007385 MUT) was constructed. (b and c) Dual‐luciferase reporter assay evaluated relative luciferase activity of circ_0007385 WT/MUT in A549 and H1975 cells transfected with miR‐519d‐3p mimic (miR‐519d‐3p) or miR‐NC mimic (miR‐NC) () miR‐NC, () miR‐519d‐3p; () miR‐NC, () miR‐519d‐3p. (d) RT‐qPCR detected relative expression of miR‐519d‐3p in tissues in the Normal (n = 75) and Tumor (n = 75) groups. (e) Pearson correlation coefficient (r) analysis determined the correlation between circ_0007385 and miR‐519d‐3p expression in NSCLC tumors (n = 75). (f and g) RT‐qPCR detected miR‐519d‐3p level in (f) 16HBE, A549 and H1975 cells, and (g) A549 and H1975 cells transfected with sh‐circ or sh‐NC () sh‐NC, () sh‐circ. **P < 0.01.

Inline graphic — The target relationship between circ_0007385 and miRNA (miR)‐519d‐3p. (a) The StarBase algorithms showed the complementary binding sequence of miR‐519d‐3p on the wild‐type of circ_0007385 (circ_0007385 WT). The mutant type of circ_0007385 (circ_0007385 MUT) was constructed. (b and c) Dual‐luciferase reporter assay evaluated relative luciferase activity of circ_0007385 WT/MUT in A549 and H1975 cells transfected with miR‐519d‐3p mimic (miR‐519d‐3p) or miR‐NC mimic (miR‐NC) () miR‐NC, () miR‐519d‐3p; () miR‐NC, () miR‐519d‐3p. (d) RT‐qPCR detected relative expression of miR‐519d‐3p in tissues in the Normal (n = 75) and Tumor (n = 75) groups. (e) Pearson correlation coefficient (r) analysis determined the correlation between circ_0007385 and miR‐519d‐3p expression in NSCLC tumors (n = 75). (f and g) RT‐qPCR detected miR‐519d‐3p level in (f) 16HBE, A549 and H1975 cells, and (g) A549 and H1975 cells transfected with sh‐circ or sh‐NC () sh‐NC, () sh‐circ. **P < 0.01.