Figure 5.
The contribution of miR‐519d‐3p to the effect of circ_0007385 knockdown in NSCLC cells in vitro. (a) RT‐qPCR measured miR‐519d‐3p level in A549 and H1975 cells transfected with miR‐519d‐3p inhibitor (anti‐miR‐519d‐3p) or its control miR‐NC inhibitor (anti‐NC) (
) anti‐NC, (
) anti‐miR‐519d‐3p. (b–j) A549 and H1975 cells were transfected with sh‐circ or sh‐NC, and cotransfected with sh‐circ and anti‐miR‐519d‐3p, or sh‐circ and anti‐NC. (b and c) CCK‐8 assessed OD450 nm value on 24, 48 and 72 hours (
) sh‐NC, (
) sh‐circ, (
) sh‐circ+anti‐NC, (
) sh‐circ+anti‐miR‐519d‐3p; () sh‐NC, () sh‐circ, () sh‐circ+anti‐NC, () sh‐circ+anti‐miR‐519d‐3p. (d and e) Transwell assays determined the numbers of migratory and invasive cells () sh‐NC, () sh‐circ, (
) sh‐circ+anti‐NC, (
) sh‐circ+anti‐miR‐519d‐3p; () sh‐NC, () sh‐circ, () sh‐circ+anti‐NC, () sh‐circ+anti‐miR‐519d‐3p. (f) Western blotting detected protein expression of the PCNA, E‐cad and N‐cad () sh‐NC, () sh‐circ, () sh‐circ+anti‐NC, () sh‐circ+anti‐miR‐519d‐3p; () sh‐NC, () sh‐circ, () sh‐circ+anti‐NC, () sh‐circ+anti‐miR‐519d‐3p. (g–j) A549 and H1975 cells transfected with sh‐circ or sh‐NC were treated with DDP for 48 hours. (g) CCK‐8 measured IC50 of DDP after treatment of 0, 2.5, 5, 10, 20, 40, and 80 μM of DDP () sh‐NC, () sh‐circ, () sh‐circ+anti‐NC, () sh‐circ+anti‐miR‐519d‐3p. (h) FCM examined apoptosis rate (%) () sh‐NC, () sh‐circ, () sh‐circ+anti‐NC, () sh‐circ+anti‐miR‐519d‐3p and (i and j) caspase‐3 activity after treatment of 10 μM of DDP () sh‐NC, () sh‐circ, () sh‐circ+anti‐NC, () sh‐circ+anti‐miR‐519d‐3p; () sh‐NC, () sh‐circ, () sh‐circ+anti‐NC, () sh‐circ+anti‐miR‐519d‐3p. **P < 0.01.