Fig. 1.

The IGFBP‐3 peptide has less liposome disruption capability compared to the CDT control. Liposomes were prepared as described in Materials and methods followed by addition of the IGFBP peptides. Negative controls contained 5% DMSO/PBS, which was predetermined not to cause dye leakage, while Triton X‐100 and CDT were used as positive controls. After a 10‐min period, fluorescence values of the samples in 96‐well plates were measured by a spectrofluorometer (filter set to 485 nm excitation and 528 nm emission). Fluorescence measurements indicate dye leakage corresponding to liposome damage. Triton X‐100 detergent (10% v/v in PBS) was used as the positive control for determination of 100% leakage. Percent liposome dye leakage was calculated, and each column represents the mean ± SD of three independent experiments, each run in triplicate. The asterisks (*P < 0.05, **P < 0.01) indicate a statistically significant difference from the control. The absence of asterisks indicates no significance, Mann–Whitney test.