Fig. 4.

Binding of biotin‐HN or biotin‐HA to IGFBP‐3 is not altered by IGFBP‐3 glycosylation. Binding of biotin‐HN or biotin‐HA to immobilized glycosylated or nonglycosylated IGFBP‐3 was measured by ELISA. IGFBP‐3 (50 nm) was bound to the wells; then, increasing concentrations of biotin‐HN or biotin‐HA were added and processed as described in the Materials and methods. The curves were drawn using the graphpad prism 8.4.2 software. Data were expressed as the mean ± SD of three independent experiments.