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. 2020 Jul 21;7:139. doi: 10.3389/fmolb.2020.00139

Figure 2.

Figure 2

Effect of imaging force on vesicle images. (A) Topography, peak force error and deformation image recorded at 100 pN. (B) Topography, peak force error and deformation image recorded at 250 pN. All scale bars are 100 nm. (C) Comparison of line profiles through the maximum of the vesicles recorded at 100 and 250 pN. The dashed red line indicates the approximate height expected for a double lipid bilayer (~10 nm). Upper panel corresponds to the data in (A,B). (D) Height, radius of curvature and full width at half maximum (FWHM) of liposomes recorded at various imaging forces. At least 180 particles were analyzed for each condition; error bars indicating standard error of the mean fall within the marker size. Lines show linear fits with slopes:−0.020 (Height), −0.056 (FWHM) and −0.053 (Rc).