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. 2020 Jul 17;7:429. doi: 10.3389/fvets.2020.00429

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Copyright © 2020 Morell, Vogl, IJsseldijk, Piscitelli-Doshkov, Tong, Ostertag, Ferreira, Fraija-Fernandez, Colegrove, Puel, Raverty and Shadwick.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunofluorescence images from maximum projections from z stacks from several locations of the cochlea of (a–d) Seba's short-tailed bat (Carollia perspicillata) and (e) Mustached bat (Pteronotus parnellii). We labeled the whole mounts with anti-prestin antibody from Santa Cruz (SC) in (a,e) (green, 1:100 for Seba's short tailed bat and 1:200 for Mustached bat), with anti-prestin antibody from Dr. Zheng lab (green) in (c,d), anti-myosinVI antibody (red) that labels inner (IHCs) and outer hair cells (OHCs), and DAPI (blue). (b) Specificity control, secondary control (control for non-specific binding of the secondary antibodies) and autofluorescence control from (a). The cuticular plate from IHCs and OHCs was positively labeled by anti-prestin antibody from Santa Cruz, but not with anti-prestin antibody from Dr. Zheng lab. The animal image inserted in (a) corresponds to the animal species of cochlea stained by immunofluorescence.