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. 2020 Jul 23;7:439. doi: 10.3389/fvets.2020.00439

Figure 4.

Figure 4

Radioresistant cell lines have modified basal proliferation rates relative to their parental cells. (A) SRB assays showing differences in proliferation rates between MCF-7, ZR-751, MDA-MB-231, and REM-134 cell lines and their derived RR cell lines grown in 2D cultures (2-way ANOVA with Holm-Šídák multiple comparisons test; data expressed as mean ± SEM, n = 3, ****p ≤ 0.0001; ***p ≤ 0.001). (B) Heatmap showing log2 mean-centered gene expression profiles of proliferation genes in parental and RR cell lines showing key G1/S phase regulators taken from the KEGG database cell cycle pathway (55); red = higher expression, black = no change, green = lower expression. Heatmap clustering was carried out using Pearson correlation with average linkage. The gene list is shown in Supplementary Table 3. (Ci) IHC of MTS stained for Ki67 using MCF-7, ZR-751, and REM-134 parental and RR cell lines. (Cii) Quantitative analysis of the % of cells with Ki67 staining (unpaired, two tailed t-test; data expressed as mean ± SEM, n = 3, ****p ≤ 0.0001).