Table 1.
Primary antibodies used for western blotting (WB), immunocytochemistry (ICC), and immunohistochemistry (IHC).
| Primary antibody target antigen | Antibody details | Dilutions | Antigen retrieval |
|---|---|---|---|
| Anti-ERα | Mouse mAb; Dako; M7047 | 1:50 (ICC, IHC) | Sodium citrate |
| Anti-HER2 | Rabbit mAb; Cell signaling technology; 2,242 | 1:50 (ICC, IHC) | Sodium citrate |
| Anti-PR | Mouse mAb; Dako; M3569 | 1:150 (ICC, IHC) | EDTA |
| Anti-AKT | Mouse mAb; Cell signaling technology; 2,920 | 1:1000 (WB) | N/A |
| Anti-Phospho AKT | Rabbit pAb; Cell signaling technology; 9,271 | 1:1000 (WB) | N/A |
| Anti-ERK | Rabbit pAb; Cell signaling Technology; 9,102 | 1:1000 (WB) | N/A |
| Anti-Phospho ERK | Mouse mAb; Cell signaling technology; 9,106 | 1:1000 (WB) | N/A |
| Anti-ki67 | Rabbit mAb; Abcam; 92,742 | 1:150 (ICC, IHC) | Sodium citrate |
| Anti-hypoxyprobe-1 | Mouse mAb; Hypoxyprobe; HP1-100Kit | 1:2000 (IHC) | Sodium citrate |
| Anti-E-cadherin | Mouse mAb; BD transduction; 610,182 | 1:50 (ICC, IHC) | Sodium citrate |
| Anti-N-cadherin | Mouse mAb; BD transduction; 610,921 | 1:150 (ICC, IHC) | Sodium citrate |
| Anti-vimentin | Mouse mAb; Abcam; 8,069 | 1:50 (ICC, IHC) | Sodium citrate |
| Anti-SNAIL | Rabbit pAb; Abcam; 128,530 | 1:250 (ICC, IHC) | Sodium citrate |
| Anti-WNT5a | Mouse mAb; Thermo Scientific; MA5-15,502 | 1:500 (IHC) | Sodium citrate |
| Anti-Frizzled | Mouse pAb; R&D Systems; AF1120 | 1:50 (IHC) | Sodium citrate |