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. 2020 Jul 22;11:1380. doi: 10.3389/fimmu.2020.01380

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Copyright © 2020 Han, Zhang, Xu, Pang, Zhang, Hu, Chen, Chen, Zhang and He.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cecum contents from ATB-treated mice enhance the cytotoxicity of γδT cells in vitro. (A) In vitro cytotoxicity of γδT cells against HepG2 hepatocellular carcinoma cells. Fecal extracts (ex) from different groups of mice or an equal volume of PBS were added into a γδT cell and HepG2 co-culture system for 4 h. Here, the results of four experiments are shown. Analysis of variance (ANOVA), **p < 0.01, and ***p < 0.001, ****p < 0.0001. (B) The same methods described in (A) were used to detect the cytotoxicity of γδT cells against NCI-H446 tumor cells and NCI-H520 tumor cells (C). (D) Perforin secreted in the supernatant of γδT cells cultured with fecal extracts or PBS. *p < 0.05 and ***p < 0.001 (ANOVA). (E) Granzyme A detected with the same method in (D). ns, no significant. (F) Granzyme B detected with the same method in (D). The data represent three independent experiments. *P < 0.05 and (ANOVA).