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. 2020 Jul 22;11:1380. doi: 10.3389/fimmu.2020.01380

Figure 6.

Figure 6

3-Indolepropionic acid enhances the cytotoxicity of γδT cells both in vitro and in vivo. (A) 3-Indopropionic acid (IPA) or an equal volume of phosphate-buffered saline (PBS) was added to a γδT cell and HepG2 co-culture system for 4 h, and the cytotoxicity of γδT cells was detected by using a Cytototo96 kit. The plot shows four independent experiments. ***p < 0.001 (unpaired t-test). (B) HepG2 cells were cultured in 96-well plates for 72 h, 10,000 cells per well and 5 μM IPA, 10 μM IPA, or an equal volume of medium were added to examine proliferation and viability by MTT (cell proliferation kit 1). Proliferation was calculated as the measured value/original value. (C) Tumor growth of HepG2-bearing nude mice treated with or without (PBS control) IPA three times or IPA combined with γδT cell therapy. Data are the mean ± SEM of the tumor sizes for six mice in each group. *p < 0.05 (ANOVA). (D) Image of the tumor from the mice in (C).