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. 2020 Jul 17;11:461. doi: 10.3389/fendo.2020.00461

Figure 1.

Figure 1

Ovarian follicle cells of mature eggs express dilp8/DILP8. (A) Using a 10X-GFP the dilp8-Gal4 expression is strong in follicle cells surrounding mature eggs (stage 14). (B) DILP8 antiserum labels follicle cells in mature eggs (stage 14) in a wild type fly (Cs, Canton s). (C) A dilp8 mutant fly (dilp8MI00727) with an eGFP-insertion reveals GFP expression in follicle cells. The sample was also incubated in anti-DILP8. (D) DILP8 antiserum yields no immunolabeling in the same specimen as in (C), due to the dilp8 mutation. Asterisks label the same two eggs in (C,D). (E) In 1-week-old flies kept under normal conditions dilp8-Gal4 expression (dilp8 > GFP) is seen in follicle cells of stage 14 eggs [in (E–G) 10X-GFP was not used]. (F) In 3-week-old flies dilp8-Gal4 expression in mature eggs is somewhat weaker. (G) In flies kept for 3 weeks in reproductive diapause ovary development is arrested and no eggs develop into vitellogenic stages and only residual dilp8-Gal4 expression is seen in unidentified cells.