Figure 5.

Manipulations of dilp8 affect fecundity in flies. (A) dilp8 mutants (hetero- and homozygous) lay fewer eggs than controls. Fifteen to thirty flies from three replicates were used (****p < = 0.00001, one-way ANOVA followed by Tukey's test). (B) The number of eggs retained in the flies is significantly higher in homozygous dilp8 mutants. Nine to twenty-seven flies from three replicates were used (****p < 0.0001, one-way ANOVA followed by Tukey's test). (C) The higher number of eggs retained in dilp8 mutant flies is reflected in the bloated abdomen (not seen in heterozygote or control). (D) Knockdown of dilp8 using dilp8-Gal4 significantly reduces egg laying over 48 h, whereas dilp8 overexpression has no effect (*p < 0.05, **p < 0.01). (E) Overexpression and knockdown of dilp8 in follicle cells (FC2-Gal4) does not significantly affect egg laying over 48 h. Twenty-one to thirty flies from three replicates were used (one-way ANOVA followed by Tukey's test). (F) Knockdown of dilp8 in follicle cells leads to a higher number of eggs retained in flies, but overexpression has no effect [20–28 flies from three replicates were used (**p < 0.01, one-way ANOVA followed by Tukey's test)].