FIGURE 3.

Genetic engineering of the mevalonate pathway. (A) Graphical representation of the genetic modification at the ip^s locus encoding an iron sulfur protein conferring carboxin resistance. The wild type (wt) version encoding a sensitive version ip^s is given in dark gray. The corresponding resistant version is given in light gray (ip^r). The gene of interest (GOI) and fragments expected in Southern blot analysis (shown in B) are given in red and blue, respectively. The probe used for hybridization is indicated as a green.