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. 2020 Jul 23;12:28. doi: 10.3389/fnsyn.2020.00028

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Copyright © 2020 Aparicio, Formoso, León, Frasch and Scorticati.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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GRAPHICAL ABSTRACT — Sunburst plot from SynGO analysis of M6a’s potential synaptic interactors (https://www.syngoportal.org/, Koopmans et al., 2019). This work shows a combination of co-immunoprecipitation assay with quantitative tandem mass tag spectrometry (TMT/MS) to identify potential protein-protein interactions. Using a chimera protein that only contains the extracellular loops of neuronal glycoprotein M6a, we performed a co-IP with rat hippocampi samples followed by TMT/MS. Data analysis revealed 72 potential interactors of M6a’s loops, 45 of which are related to synapse localization.