Table 1.

Primers and annealing temperatures used to detect the carriage of virulence and resistance genes in the current study.

Gene	Primers	Annealing temperature	Product size	References
lukS	fw: 5′-TGTAAGCAGCAGAAAATGGGG-3′rev: 5′-GCCCGATAGGACTTCTTACAA-3′	57°C	503 bp	(24)
lukF	fw: 5′-CCTGTCTATGCCGCTAATCAA-3′rev: 5′-AGGTCATGGAAGCTATCTCGA-3′	57°C	572 bp
siet	fw:5′-ATGGAAAATTTAGCGGCATCTGG-3′rev:5′-CCATTACTTTTCGCTTGTTGTGC-3′	56°C	359 bp	(25)
seccanine	fw:5′-GGGAAGCTTGTAATTTTGATATTCGCACT-3′rev:5′- CCCGGATCCTATCAAAATCGGATTAACA-3′	40°C	800 bp	(28)
expA	fw: 5′-GCGCGTCCTTCTGATCCAGAACT-3′rev: 5′-AACGTCCCCCTTTACCTACGTGAAT-3′	58°C	574 bp	(26)
expB	fw: 5′-GGGCATGCACATATGATGAAGCC-3′rev: 5′-CCAGATCTATCTTCTGATTCAGC-3′	50°C	843 bp	(27)
fusB	fw: 5′-CCGTCAAAGTTATTCAATCG-3′rev: 5′-ACAATGAATGCTATCTCGACA-3′	50°C	492 bp	(9)
fusC	fw: 5′-GGACTTTATTACATCGATTGAC-3′rev: 5′-CTGTCATAACAAATGTAATCTCC-3′	50°C	411 bp
fusD	fw: 5′-AATTCGGTCAACGATCCC-3′rev: 5′-GCCATCATTGCCAGTACG-3′	57°C	465 bp
ileS	fw: 5′-TATATTATGCGATGGAAGGTTGG-3′rev: 5′-AATAAAATCAGCTGGAAAGTGTTG-3′	57°C	458 bp	(21)
smr	fw: 5′-ATAAGTACTGAAGTTATTGGAAGT-3′rev: 5′-TTCCGAAAATGTTTAACGAAACTA-3′	48°C	285 bp	(23)
qac A/B	fw: 5′-GCTGCATTTATGACAATGTTTG-3′rev: 5′-AATCCCACCTACTAAAGCAG-3′	40°C	628 bp
icaA	fw: 5′-CCTAACTAACGA AAGGTA G-3′ rev: 5′-AAG ATATAGCGA TAA GTG C-3′	49°C	1315 bp	(30)
icaD	fw: 5′-AAA CGT AAG AGA GGT GG-3′ rev: 5′-GGC AAT ATG ATC AAG ATA C-3′	49°C	381 bp
bap	fw: 5′- CCCTATATCGAAGGTGTAGAATTG-3′rev: 5′-GCTGTTGAAGTTAATACTGTACCTGC-3′	62°C	971 bp	(29)

fw, forward primer; rev, reverse primer; bp, base pair.