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. 2020 Jul 23;8:670. doi: 10.3389/fcell.2020.00670

FIGURE 1.

FIGURE 1

Knockdown of DDHD1 induces enhanced neurite outgrowth in SH-SY5Y cells. (A) SH-SY5Y cells treated with luciferase siRNA (control siRNA), DDHD1 siRNA#2, or DDHD1 siRNA#5 were analyzed by Western blotting with the indicated antibodies. (B) At 72 h after siRNA transfection, SH-SY5Y cells were subjected to RA treatment for 72 h to induce neurite outgrowth. The cells were fixed and stained with TRITC-phalloidin. Scale bars, 50 μm. (C,D) Quantification of the data in (B). The graph shows the average length of the longest neurite tubule (C) and the number of neurite branches (D) in each cell. (E) SH-SY5Y cells were treated with DDHD1 siRNA#5 or control siRNA for 48 h, and then infected with the indicated retroviruses. At 24 h after infection, the cells were treated with RA for 72 h. The fluorescent signals for FITC-phalloidin, mCherry and merged image are shown. Scale bars, 50 μm. (F,G) Quantification of the data in (E). The average length of the longest neurite tubule (F) and the number of neurite branches (G) in each cell was measured and is shown in the graph. At least 50 cells were measured in each experiment. Statistic values are expressed as means for three independent experiments ± S.D. *p < 0.05; **p < 0.01; ***p < 0.001 (Tukey test).